Application
Western blot analysis of AUP1 against human AUP1 (AA: 229-410) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-17 against HEK293 (1) and AUP1 (AA: 229-410)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-17 against A431 (1), NIH/3T3 (2), Hela (3), SW480 (4), CHO3D10 (5), A549 (6), and SPC-A-1 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | AUP1 [1H6A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AA589454 antibody Ancient ubiquitous protein 1 antibody AUP 1 antibody aup1 antibody AUP1_HUMAN antibody |
| Product description | The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human AUP1 (AA: 229-410) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/mL |
| Alternative Names | AA589454 antibody Ancient ubiquitous protein 1 antibody AUP 1 antibody aup1 antibody AUP1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 53kDa |
| Cellular Localization | Endoplasmic reticulum membrane. |
| SwissProt ID | Q9Y679 |
|---|
Application
Western blot analysis of AUP1 against human AUP1 (AA: 229-410) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-17 against HEK293 (1) and AUP1 (AA: 229-410)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-17 against A431 (1), NIH/3T3 (2), Hela (3), SW480 (4), CHO3D10 (5), A549 (6), and SPC-A-1 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | A431, NIH/3T3, Hela, SW480, CHO3D10, A549, and SPC-A-1 cell |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
