Application
Western blot analysis of LILRA3 against human LILRA3 (AA: 24-168) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-19, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-19 against HEK293 (1) and LILRA3 (AA: 24-168)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-19, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of LILRA3 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-19, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | LILRA3 [6F12D7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 85E antibody CD85 antigen-like family member E antibody CD85e antibody CD85e antigen antibody e3 antibody HM 31 antibody HM 43 antibody HM31 antibody HM43 antibody ILT 6 antibody ILT-6 antibody ILT6 antibody Immunoglobulin like transcript 6 antibody Immunoglobulin-like transcript 6 antibody Leucocyte immunoglobulin like receptor antibody Leukocyte immunoglobulin like receptor 4 antibody Leukocyte immunoglobulin like receptor A3 antibody Leukocyte immunoglobulin like receptor subfamily A (without TM domain) member 3 antibody Leukocyte immunoglobulin like receptor subfamily A member 3 antibody Leukocyte immunoglobulin-like receptor 4 antibody Leukocyte immunoglobulin-like receptor subfamily A member 3 antibody LILRA 3 antibody LILRA3 antibody LIR 4 antibody LIR-4 antibody LIR4 antibody LIRA3_HUMAN antibody Monocyte inhibitory receptor HM43/HM31 antibody |
| Product description | This gene encodes a member of a family of immunoreceptors that are expressed predominantly in monocytes and B cells, and at lower levels in dendritic cells and natural killer cells. The encoded protein lacks the transmembrane region found in other members of this family. It acts as a soluble receptor for class I major histocompatibility complex (MHC) antigens. Alternatively spliced transcript variants encoding different isoforms have been found. This gene is located in a cluster of related genes on chromosome 19 and is polymorphic in human populations, with many individuals containing a deletion of this genomic region. |
| Immunogen | Purified recombinant fragment of human LILRA3 (AA: 24-168) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 85E antibody CD85 antigen-like family member E antibody CD85e antibody CD85e antigen antibody e3 antibody HM 31 antibody HM 43 antibody HM31 antibody HM43 antibody ILT 6 antibody ILT-6 antibody ILT6 antibody Immunoglobulin like transcript 6 antibody Immunoglobulin-like transcript 6 antibody Leucocyte immunoglobulin like receptor antibody Leukocyte immunoglobulin like receptor 4 antibody Leukocyte immunoglobulin like receptor A3 antibody Leukocyte immunoglobulin like receptor subfamily A (without TM domain) member 3 antibody Leukocyte immunoglobulin like receptor subfamily A member 3 antibody Leukocyte immunoglobulin-like receptor 4 antibody Leukocyte immunoglobulin-like receptor subfamily A member 3 antibody LILRA 3 antibody LILRA3 antibody LIR 4 antibody LIR-4 antibody LIR4 antibody LIRA3_HUMAN antibody Monocyte inhibitory receptor HM43/HM31 antibody |
|---|---|
| Molecular Weight(MW) | 47.5kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q8N6C8 |
|---|
Application
Western blot analysis of LILRA3 against human LILRA3 (AA: 24-168) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-19, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-19 against HEK293 (1) and LILRA3 (AA: 24-168)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-19, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of LILRA3 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-19, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
