Application
Western blot analysis of CD87 against human CD87 (AA: 23-305) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-25 against HEK293 (1) and CD87 (AA: 23-305)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD87 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-25, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD87 [1D1B11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 87 antibody CD87 antibody CD87 antigen antibody MO 3 antibody MO3 antibody Monocyte activation antigen Mo3 antibody Plasminogen activator receptor urokinase antibody Plasminogen activator urokinase receptor antibody PLAUR antibody U PAR antibody u plasminogen activator receptor antibody U-PAR antibody u-plasminogen activator receptor form 2 antibody UPA receptor antibody uPAR antibody UPAR_HUMAN antibody Urinary plasminogen activator receptor antibody URKR antibody Urokinase plasminogen activator receptor antibody Urokinase plasminogen activator surface receptor antibody urokinase-type plasminogen activator (uPA) receptor antibody |
| Product description | This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. |
| Immunogen | Purified recombinant fragment of human CD87 (AA: 23-305) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 87 antibody CD87 antibody CD87 antigen antibody MO 3 antibody MO3 antibody Monocyte activation antigen Mo3 antibody Plasminogen activator receptor urokinase antibody Plasminogen activator urokinase receptor antibody PLAUR antibody U PAR antibody u plasminogen activator receptor antibody U-PAR antibody u-plasminogen activator receptor form 2 antibody UPA receptor antibody uPAR antibody UPAR_HUMAN antibody Urinary plasminogen activator receptor antibody URKR antibody Urokinase plasminogen activator receptor antibody Urokinase plasminogen activator surface receptor antibody urokinase-type plasminogen activator (uPA) receptor antibody |
|---|---|
| Molecular Weight(MW) | 37kDa |
| Cellular Localization | Secreted and Cell membrane. |
| SwissProt ID | Q03405 |
|---|
Application
Western blot analysis of CD87 against human CD87 (AA: 23-305) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-25 against HEK293 (1) and CD87 (AA: 23-305)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD87 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-25, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
