Application
Western blot analysis of CHRM3 against human CHRM3 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-27 against HEK293 (1) and CHRM3 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CHRM3 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-27, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CHRM3 [4A6B7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AChR antibody ACM3_HUMAN antibody cholinergic receptor muscarinic 3 antibody CHRM 3 antibody CHRM3 antibody EGBRS antibody HM 3 antibody HM3 antibody m3 muscarinic acetylcholine receptor antibody M3 muscarinic receptor antibody muscarinic 3 antibody Muscarinic acetylcholine receptor M3 antibody muscarinic cholinergic m3 receptor antibody muscarinic m3 receptor antibody |
| Product description | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. |
| Immunogen | Purified recombinant fragment of human CHRM3 (AA: extra mix) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | AChR antibody ACM3_HUMAN antibody cholinergic receptor muscarinic 3 antibody CHRM 3 antibody CHRM3 antibody EGBRS antibody HM 3 antibody HM3 antibody m3 muscarinic acetylcholine receptor antibody M3 muscarinic receptor antibody muscarinic 3 antibody Muscarinic acetylcholine receptor M3 antibody muscarinic cholinergic m3 receptor antibody muscarinic m3 receptor antibody |
|---|---|
| Molecular Weight(MW) | 66kDa |
| Cellular Localization | Cell membrane. Cell junction. |
| SwissProt ID | P20309 |
|---|
Application
Western blot analysis of CHRM3 against human CHRM3 (AA: extra mix) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-27 against HEK293 (1) and CHRM3 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CHRM3 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-27, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Jurkat cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
