Application
Western blot analysis of CD96 against human CD96 (AA: extra 321-519) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-31 against HEK293 (1) and CD96 (AA: extra 321-519)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-31 againstHela (1), Jurkat (2), MOLT4 (3), Raji (4), HL-60 (5), U937 (6), and C6 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD96 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-31, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD96 [5E6C12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD96 antibody CD96 molecule antibody Cell surface antigen CD96 antibody DKFZp667E2122 antibody MGC22596 antibody T cell activated increased late expression protein antibody T cell activation, increased late expression antibody T cell surface protein tactile precursor antibody T cell-activated increased late expression protein antibody T-cell surface protein tactile antibody TACT_HUMAN antibody Tactile antibody |
| Product description | CD96 (Cluster of Differentiation 96) or Tactile (T cell activation, increased late expression) is a receptor expressed on T cells and NK cells, and shares sequence similarity with CD226 (also known as DNAM-1). The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesion of activated T and NK cells to their target cells during the late phase of the immune response. It may also function in antigen presentation[citation needed]. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. CD96 is a transmembrane glycoprotein that has three extracellular immunoglobulin-like domains and is expressed by all resting human and mouse NK cells. CD96 main ligand is CD155. CD 96 has approximately 20% homology with CD226 and competed for binding to CD155 with CD226. |
| Immunogen | Purified recombinant fragment of human CD96 (AA: extra 321-519) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/mL |
| Alternative Names | CD96 antibody
CD96 molecule antibody
Cell surface antigen CD96 antibody
DKFZp667E2122 antibody
MGC22596 antibody
T cell activated increased late expression protein antibody
T cell activation increased late expression antibody T cell surface protein tactile precursor antibody T cell-activated increased late expression protein antibody T-cell surface protein tactile antibody TACT_HUMAN antibody Tactile antibody |
|---|---|
| Molecular Weight(MW) | 65.6kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P40200 |
|---|
Application
Western blot analysis of CD96 against human CD96 (AA: extra 321-519) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-31 against HEK293 (1) and CD96 (AA: extra 321-519)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-31 againstHela (1), Jurkat (2), MOLT4 (3), Raji (4), HL-60 (5), U937 (6), and C6 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD96 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-31, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela, Jurkat, MOLT4, Raji, HL-60, U937, and C6 cell K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
