Application
Western blot analysis of PSG1 against human PSG1 (AA: 250-419) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-36 against HEK293 (1) and PSG1 (AA: 250-419)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of PSG1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-36, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | PSG1 [5C6G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The human placenta is a multihormonal endocrine organ that produces hormones, enzymes, and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG, PSG) is a major product of the syncytiotrophoblast, reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women (Horne et al., 1976 [PubMed 971765]). PSG is a member of the immunoglobulin (Ig) superfamily (Watanabe and Chou, 1988 [PubMed 3257488]; Streydio et al., 1988 |
| Immunogen | Purified recombinant fragment of human PSG1 (AA: 250-419) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | B1G1 antibody Carcinoembryonic antigen SG8 antibody CD66 antigen-like family member F antibody CD66f antibody CD66f antigen antibody CEA antibody DHFRP2 antibody Fetal liver non-specific cross-reactive antigen 1/2 antibody FL-NCA-1/2 antibody PBG 1 antibody PBG1 antibody Pregnancy specific B 1 glycoprotein antibody Pregnancy specific beta 1 glycoprotein 1 antibody Pregnancy specific beta 1 glycoprotein 2 antibody Pregnancy specific beta 1 glycoprotein 7 antibody Pregnancy specific beta 1 glycoprotein 8 antibody Pregnancy specific beta 1 glycoprotein antibody Pregnancy specific beta 1 glycoprotein C/D antibody Pregnancy-specific beta-1 glycoprotein C/D antibody Pregnancy-specific beta-1-glycoprotein 1 antibody Pregnancy-specific glycoprotein 1 antibody PS beta C/D antibody PS-beta-C/D antibody PS-beta-G-1 antibody PSBG 1 antibody PSBG 2 antibody PSBG-1 antibody PSBG1 antibody PSBG2 antibody PSG 1 antibody PSG 2 antibody PSG 8 antibody PSG 95 antibody PSG IIA d antibody PSG1 antibody PSG1_HUMAN antibody PSG2 antibody PSG8 antibody PSG95 antibody PSGGA antibody PSGGB antibody PSGIIA a antibody PSGIIA antibody PSGIIA b antibody PSGIIA c antibody SP 1 antibody SP1 antibody |
|---|---|
| Molecular Weight(MW) | 47.2kDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P11464 |
|---|
Application
Western blot analysis of PSG1 against human PSG1 (AA: 250-419) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-36 against HEK293 (1) and PSG1 (AA: 250-419)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of PSG1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-36, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
