Application
Western blot analysis of CD72 against human CD72 (AA: extra 117-359) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-37 against HEK293 (1) and CD72 (AA: extra 117-359)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD72 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-37, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD72 [2F3B8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B cell differentiation antigen CD72 antibody B-cell differentiation antigen CD72 antibody CD_antigen=CD72 antibody CD72 antibody CD72 antigen antibody CD72 molecule antibody CD72_HUMAN antibody CD72b antibody CD72c antibody Ly-19 antibody Ly-32 antibody Ly-m19 antibody Lyb-2 antibody Lyb2 antibody LYB2, mouse, homolog of antibody Lymphocyte antigen 32 antibody MGC108873 antibody |
| Product description | CD72 (Cluster of Differentiation 72), also known in murine biology as Lyb-2, is a protein active in the immune system of animals. It consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells (from the pro-B through the mature B-cell stage), where it appears to mediate aspects of B-cell - T-cell interaction. It is a ligand for CD5. CD72 is a regulatory protein on B lymphocytes. The cytoplasmic tail of CD72 contains two potential immunoreceptor tyrosine-based inhibitory motifs, one of which has been shown to recruit the tyrosine phosphatase SHP- 1. These features suggest a negative regulatory role for CD72. CD72 is a nonredundant regulator of B-cell development and a negative regulator of B-cell responsiveness. |
| Immunogen | Purified recombinant fragment of human CD72 (AA: extra 117-359) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | B cell differentiation antigen CD72 antibody
B-cell differentiation antigen CD72 antibody
CD_antigen=CD72 antibody
CD72 antibody
CD72 antigen antibody
CD72 molecule antibody
CD72_HUMAN antibody
CD72b antibody
CD72c antibody
Ly-19 antibody
Ly-32 antibody
Ly-m19 antibody
Lyb-2 antibody
Lyb2 antibody
LYB2 mouse homolog of antibody Lymphocyte antigen 32 antibody MGC108873 antibody |
|---|---|
| Molecular Weight(MW) | 40.2kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P21854 |
|---|
Application
Western blot analysis of CD72 against human CD72 (AA: extra 117-359) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-37 against HEK293 (1) and CD72 (AA: extra 117-359)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD72 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-37, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
