Application
Western blot analysis of BTN1A1 against human BTN1A1 (AA: extra 27-242) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-38 against HEK293 (1) and BTN1A1 (AA: extra 27-242)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-38 against Hela (1), Jurkat (2), HepG2 (3), HT-29 (4), and MCF-7 (5) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of BTN1A1 was done on K562 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-38, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | BTN1A1 [4B11B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BT antibody BT1A1_HUMAN antibody BTN antibody BTN1 antibody Btn1a1 antibody butyrophilin antibody Butyrophilin subfamily 1 member A1 antibody butyrophilin, subfamily 1, member A1 antibody |
| Product description | Butyrophilin subfamily 1 member A1 is a protein that in humans is encoded by the BTN1A1 gene. Butyrophilin (BTN) is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families. Btn1a1 regulates the amount of lipids and size of droplets expressed in milk. When the gene is compromised in laboratory mice, approximately half the pups died within the first 20 days and the remainder were significantly under-weight. Butyrophilin has been presented as a potential antigen which may be similar enough to myelin oligodendrocyte glycoprotein (MOG) to spur the immune system to attack myelin in a process known as molecular or epitopic mimicry. This suggests that ingestion of butyrophilin in dairy products from cows and goats may be a potential trigger for multiple sclerosis. Independent studies by a group in Germany have reached similar conclusions. The German group have used heavy doses of butyrophilin on mice with an experimental model for multiple sclerosis called EAE. They have found that this strategy, called immune tolerance, reduces the effects of the disease. |
| Immunogen | Purified recombinant fragment of human BTN1A1 (AA: extra 27-242) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | BT antibody
BT1A1_HUMAN antibody
BTN antibody
BTN1 antibody
Btn1a1 antibody
butyrophilin antibody
Butyrophilin subfamily 1 member A1 antibody
butyrophilin subfamily 1 member A1 antibody |
|---|---|
| Molecular Weight(MW) | 58.9kDa |
| Cellular Localization | Membrane. Secreted. |
| SwissProt ID | Q13410 |
|---|
Application
Western blot analysis of BTN1A1 against human BTN1A1 (AA: extra 27-242) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-38 against HEK293 (1) and BTN1A1 (AA: extra 27-242)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-38 against Hela (1), Jurkat (2), HepG2 (3), HT-29 (4), and MCF-7 (5) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of BTN1A1 was done on K562 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-38, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela, Jurkat, HepG2, HT-29, and MCF-7 cell K562 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
