Application
Western blot analysis of CD49D against human CD49D (AA: extra 833-977)) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-40 against HEK293 (1) and CD49D (AA: extra 833-977))-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-40 against HL-60 (1) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD49D was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-40, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD49D [7E4C5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 269C wild type antibody Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody CD49 antigen like family member D antibody CD49 antigen-like family member D antibody CD49d antibody IA4 antibody Integrin alpha 4 antibody Integrin alpha 4 subunit antibody Integrin alpha IV antibody Integrin alpha-4 antibody Integrin alpha-IV antibody Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody ITA4_HUMAN antibody ITGA4 antibody MGC90518 antibody OTTHUMP00000205320 antibody Very late activation protein 4 receptor, alpha 4 subunit antibody VLA 4 subunit alpha antibody VLA-4 subunit alpha antibody VLA4 antibody |
| Product description | The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD49D (AA: extra 833-977) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | 269C wild type antibody
Antigen CD49D alpha 4 subunit of VLA 4 receptor antibody CD49 antigen like family member D antibody CD49 antigen-like family member D antibody CD49d antibody IA4 antibody Integrin alpha 4 antibody Integrin alpha 4 subunit antibody Integrin alpha IV antibody Integrin alpha-4 antibody Integrin alpha-IV antibody Integrin alpha 4 (antigen CD49D alpha 4 subunit of VLA 4 receptor) antibody ITA4_HUMAN antibody ITGA4 antibody MGC90518 antibody OTTHUMP00000205320 antibody Very late activation protein 4 receptor alpha 4 subunit antibody VLA 4 subunit alpha antibody VLA-4 subunit alpha antibody VLA4 antibody |
|---|---|
| Molecular Weight(MW) | 114.9kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P13612 |
|---|
Application
Western blot analysis of CD49D against human CD49D (AA: extra 833-977)) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-40 against HEK293 (1) and CD49D (AA: extra 833-977))-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-40 against HL-60 (1) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD49D was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-40, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
