Application
Western blot analysis of CD351 against human CD351 (AA: extra 221-450) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-45 against HEK293 (1) and CD351 (AA: extra 221-450)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD351 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-45, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD351 [5B1D12E1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FCAMR as a receptor for the Fc fragment of IgA and IgM. Binds IgA and IgM with high affinity and mediates their endocytosis. May function in the immune response to microbes mediated by IgA and IgM. Fcα/μR, also known as is CD351 (Cluster of Differentiation 351), is an Fc receptor that binds IgM with high affinity and IgA with a 10-fold lower affinity.[1] In mice the receptor is expressed on macrophages, follicular dendritic cells, marginal zone and follicular B cells and kidney tubular epithelial cells. In humans expressed has been described on intestinal lamina propria cells, Paneth cells, follicular dendritic cells in tonsils, activated macrophages and some types of pre-germinal centre IgD+/CD38+ B cells. |
| Immunogen | Purified recombinant fragment of human CD351 (AA: extra 221-450) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | CD351 antibody
Fc alpha/mu receptor antibody
Fc receptor IgA IgM high affinity antibody Fcamr antibody FCAMR_HUMAN antibody FKSG87 antibody High affinity immunoglobulin alpha and immunoglobulin mu Fc receptor antibody Immunity related factor antibody Receptor for Fc fragment of IgA and IgM antibody |
|---|---|
| Molecular Weight(MW) | 57.1kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q8WWV6 |
|---|
Application
Western blot analysis of CD351 against human CD351 (AA: extra 221-450) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-45 against HEK293 (1) and CD351 (AA: extra 221-450)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD351 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-45, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
