Application
Western blot analysis of CD204 against human CD204 (AA: extra 275-451) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-47 against HEK293 (1) and CD204 (AA: extra 275-451)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-47 against PC-2 (1), A549 (2), HepG2 (3), L-02 (4), and PANC-1 (5) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD204 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-47, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD204 [5B8H4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD204 antibody CD204 antigen antibody CD24 antibody Macrophage acetylated LDL receptor I and II antibody Macrophage scavenger receptor 1 antibody Macrophage scavenger receptor type III antibody Macrophage scavenger receptor types I and II antibody Msr 1 antibody MSR1 antibody MSRE_HUMAN antibody phSR1 antibody phSR2 antibody SCARA 1 antibody SCARA1 antibody Scavenger receptor class A member 1 antibody Scavenger receptor class A, member 1 antibody Scavenger receptor type A antibody Scvr antibody SR A antibody SRA antibody |
| Product description | This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. |
| Immunogen | Purified recombinant fragment of human CD204 (AA: extra 275-451) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD204 antibody
CD204 antigen antibody
CD24 antibody
Macrophage acetylated LDL receptor I and II antibody
Macrophage scavenger receptor 1 antibody
Macrophage scavenger receptor type III antibody
Macrophage scavenger receptor types I and II antibody
Msr 1 antibody
MSR1 antibody
MSRE_HUMAN antibody
phSR1 antibody
phSR2 antibody
SCARA 1 antibody
SCARA1 antibody
Scavenger receptor class A member 1 antibody
Scavenger receptor class A member 1 antibody Scavenger receptor type A antibody Scvr antibody SR A antibody SRA antibody |
|---|---|
| Molecular Weight(MW) | 49.8kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P21757 |
|---|
Application
Western blot analysis of CD204 against human CD204 (AA: extra 275-451) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-47 against HEK293 (1) and CD204 (AA: extra 275-451)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-47 against PC-2 (1), A549 (2), HepG2 (3), L-02 (4), and PANC-1 (5) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD204 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-47, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | PC-2, A549, HepG2, L-02, and PANC-1 cell HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
