Application
Western blot analysis of CD148 against human CD148 (AA: extra 36-210) recombinant protein.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-49 against HEK293 (1) and CD148 (AA: extra 36-210)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD148 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-49, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD148 [1D2C11 ] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 148 antibody CD148 antibody CD148 antigen antibody Density enhanced phosphatase 1 antibody Density-enhanced phosphatase 1 antibody DEP 1 antibody DEP-1 antibody HPTP eta antibody HPTPeta antibody Human density enhanced phosphatase 1 antibody Protein tyrosine phosphatase eta antibody Protein tyrosine phosphatase receptor type J antibody Protein tyrosine phosphatase receptor type J polypeptide antibody Protein-tyrosine phosphatase eta antibody Protein-tyrosine phosphatase receptor type J antibody PTPJ antibody Ptprj antibody PTPRJ_HUMAN antibody R PTP Eta antibody R-PTP-eta antibody R-PTP-J antibody Receptor type tyrosine protein phosphatase eta antibody Receptor-type tyrosine-protein phosphatase eta antibody SCC 1 antibody SCC1 antibody susceptibility to colon cancer 1, mouse, homolog of antibody |
| Product description | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human CD148 (AA: extra 36-210) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 148 antibody
CD148 antibody
CD148 antigen antibody
Density enhanced phosphatase 1 antibody
Density-enhanced phosphatase 1 antibody
DEP 1 antibody
DEP-1 antibody
HPTP eta antibody
HPTPeta antibody
Human density enhanced phosphatase 1 antibody
Protein tyrosine phosphatase eta antibody
Protein tyrosine phosphatase receptor type J antibody
Protein tyrosine phosphatase receptor type J polypeptide antibody
Protein-tyrosine phosphatase eta antibody
Protein-tyrosine phosphatase receptor type J antibody
PTPJ antibody
Ptprj antibody
PTPRJ_HUMAN antibody
R PTP Eta antibody
R-PTP-eta antibody
R-PTP-J antibody
Receptor type tyrosine protein phosphatase eta antibody
Receptor-type tyrosine-protein phosphatase eta antibody
SCC 1 antibody
SCC1 antibody
susceptibility to colon cancer 1 mouse homolog of antibody |
|---|---|
| Molecular Weight(MW) | 146kDa |
| Cellular Localization | Cell membrane. Cell projection. |
| SwissProt ID | Q12913 |
|---|
Application
Western blot analysis of CD148 against human CD148 (AA: extra 36-210) recombinant protein.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-49 against HEK293 (1) and CD148 (AA: extra 36-210)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD148 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-49, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
