Application
Western blot analysis of CD336 against human CD336 (AA: extra 22-192) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-52 against HEK293 (1) and CD336 (AA: extra 22-192)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD336 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-52, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD336 [7A7D4G11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Natural killer (NK) cells direct cytotoxicity against tumor or virally infected cells. NK cell-mediated cytotoxicity is stimulated by several activating receptors associated with the signaling adapter DNAX activation 12/killer cell-activating receptor-associated protein (DAP12). NKp44 is a natural cytotoxicity receptor that is is expressed on IL-2-activated human NK cells and may contribute to the increased efficiency of NK cells to mediate tumor cell lysis. NKp44 is composed of one Ig-like extracellular domain, a transmembrane segment, and a cytoplasmic domain. Prolactin up-regulates and cortisol down-regulates the surface expression of NKp44 at the transcriptional level. A cellular ligand for NKp44 (NKp44L) is expressed during HIV-1 infection and is correlated with the progression of CD4+ T cell depletion and an increase of viral load. This implicates NKp44 as a therapeutic agent that may aid in the progress towards a vaccine for HIV-1 infection. |
| Immunogen | Purified recombinant fragment of human CD336 (AA: extra 22-192) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | Activating NK receptor NKp44 antibody
CD336 antibody
CD336 antigen antibody
dJ149M18.1 antibody
LY95 antibody
Lymphocyte antigen 95 (activating NK receptor NK p44) antibody Lymphocyte antigen 95 homolog (activating NK receptor NK p44) antibody Lymphocyte antigen 95 homolog antibody Lymphocyte antigen 95 mouse homolog of antibody Natural cytotoxicity triggering receptor 2 antibody Natural killer cell p44 related protein antibody Natural killer cell p44-related protein antibody NCR2 antibody NCTR2_HUMAN antibody NK cell activating receptor (NKp44) antibody NK cell activating receptor antibody NK cell-activating receptor antibody NK p44 antibody NK-p44 antibody NKp44 antibody |
|---|---|
| Molecular Weight(MW) | 30.7kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | O95944 |
|---|
Application
Western blot analysis of CD336 against human CD336 (AA: extra 22-192) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-52 against HEK293 (1) and CD336 (AA: extra 22-192)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD336 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-52, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
