Application
Western blot analysis of CD344 against human CD344 (AA: extra 37-222) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EM1711-58 against HEK293 (1) and CD344 (AA: extra 37-222)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD344 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-58, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD344 [2F3A7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 344 antibody CD 344 antigen antibody CD344 antibody CD344 antigen antibody EVR1 antibody FEVR antibody Frizzled (Drosophila) homolog 4 antibody Frizzled 4 antibody Frizzled family receptor 4 antibody Frizzled homolog 4 (Drosophila) antibody Frizzled-4 antibody Fz 4 antibody Fz-4 antibody Fz4 antibody FZD4 antibody FZD4_HUMAN antibody FZD4S antibody FzE4 antibody GPCR antibody hFz4 antibody MGC34390 antibody WNT receptor frizzled 4 antibody |
| Product description | This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. |
| Immunogen | Purified recombinant fragment of human CD344 (AA: extra 37-222) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 344 antibody CD 344 antigen antibody CD344 antibody CD344 antigen antibody EVR1 antibody FEVR antibody Frizzled (Drosophila) homolog 4 antibody Frizzled 4 antibody Frizzled family receptor 4 antibody Frizzled homolog 4 (Drosophila) antibody Frizzled-4 antibody Fz 4 antibody Fz-4 antibody Fz4 antibody FZD4 antibody FZD4_HUMAN antibody FZD4S antibody FzE4 antibody GPCR antibody hFz4 antibody MGC34390 antibody WNT receptor frizzled 4 antibody |
|---|---|
| Molecular Weight(MW) | 60kDa |
| Cellular Localization | Membrane. Cell membrane. |
| SwissProt ID | Q9ULV1 |
|---|
Application
Western blot analysis of CD344 against human CD344 (AA: extra 37-222) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EM1711-58 against HEK293 (1) and CD344 (AA: extra 37-222)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD344 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-58, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
