Application
Western blot analysis of CD169 against human CD169 (AA: extra 20-197) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD169 against HEK293 (1) and CD169 (AA: extra 20-197)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunocytochemistry staining of CD169 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-63, 1/100) for 1 hour at room temperature, washed with PBS. Alexa FluorApplication
Flow cytometric analysis of CD169 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-63, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD169 [2A4D1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. |
| Immunogen | Purified recombinant fragment of human CD169 (AA: extra 20-197) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WB,ICC,FC |
| WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | CD169 antibody
CD169 antigen antibody
dJ1009E24.1 antibody
DKFZp667F058 antibody
FLJ00051 antibody
FLJ00055 antibody
FLJ00073 antibody
FLJ00411 antibody
FLJ32150 antibody
Sialic acid binding Ig like lectin 1 antibody
sialic acid binding Ig-like lectin 1 sialoadhesin antibody Sialic acid binding immunoglobulin like lectin 1 antibody Sialic acid-binding Ig-like lectin 1 antibody Sialoadhesin antibody Siglec 1 antibody Siglec-1 antibody Siglec1 antibody SN antibody SN_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 182.6kDa |
| Cellular Localization | Secreted and Cell membrane. |
| SwissProt ID | Q9BZZ2 |
|---|
Application
Western blot analysis of CD169 against human CD169 (AA: extra 20-197) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD169 against HEK293 (1) and CD169 (AA: extra 20-197)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of CD169 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-63, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Flow cytometric analysis of CD169 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-63, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells, Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
