Application
Western blot analysis of CD172G against human CD172G (AA: extra 29-360) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD172G against HEK293 (1) and CD172G (AA: extra 29-360)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunocytochemistry staining of CD172G in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-72, 1/100) for 1 hour at room temperature, washed with PBS. Alexa FluorApplication
Flow cytometric analysis of CD172G was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-72, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD172G [4F8C10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD172 antigen like family member B antibody bA77C3.1 antibody CD172 antigen-like family member B antibody CD172g antibody CD172g antigen antibody FLJ42230 antibody RP11-77C3.2 antibody Signal regulatory protein beta 2 antibody Signal regulatory protein gamma antibody Signal-regulatory protein beta-2 antibody Signal-regulatory protein gamma antibody SIRP B2 antibody SIRP beta 2 antibody SIRP gamma antibody SIRP-b2 antibody SIRP-beta-2 antibody SIRP-gamma antibody SIRPB2 antibody SIRPG antibody SIRPG_HUMAN antibody SIRPgamma antibody |
| Product description | The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Immunogen | Purified recombinant fragment of human CD172G (AA: extra 29-360) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD172 antigen like family member B antibody bA77C3.1 antibody CD172 antigen-like family member B antibody CD172g antibody CD172g antigen antibody FLJ42230 antibody RP11-77C3.2 antibody Signal regulatory protein beta 2 antibody Signal regulatory protein gamma antibody Signal-regulatory protein beta-2 antibody Signal-regulatory protein gamma antibody SIRP B2 antibody SIRP beta 2 antibody SIRP gamma antibody SIRP-b2 antibody SIRP-beta-2 antibody SIRP-gamma antibody SIRPB2 antibody SIRPG antibody SIRPG_HUMAN antibody SIRPgamma antibody |
|---|---|
| Molecular Weight(MW) | 42.5kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q9P1W8 |
|---|
Application
Western blot analysis of CD172G against human CD172G (AA: extra 29-360) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD172G against HEK293 (1) and CD172G (AA: extra 29-360)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of CD172G in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-72, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Flow cytometric analysis of CD172G was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-72, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells, HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
