Application
Western blot analysis of CD307C against human CD307C (AA: extra 18-153) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD307C against HEK293 (1) and CD307C (AA: extra 18-153)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD307C was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-77, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD307C [4B4B2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD307c antibody Fc receptor homolog 3 antibody Fc receptor-like protein 3 antibody FcR-like protein 3 antibody FcRH3 antibody FcRL3 antibody FCRL3_HUMAN antibody hIFGP3 antibody IFGP family protein 3 antibody IFGP3 antibody Immune receptor translocation-associated protein 3 antibody Immunoglobulin superfamily receptor translocation associated protein 3 antibody IRTA3 antibody SH2 domain-containing phosphatase anchor protein 2 antibody SPAP2 antibody |
| Product description | This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains immunoreceptor-tyrosine activation motifs and immunoreceptor-tyrosine inhibitory motifs in its cytoplasmic domain and may play a role in regulation of the immune system. Mutations in this gene have been associated with rheumatoid arthritis, autoimmune thyroid disease, and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD307C (AA: extra 18-153) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD307c antibody Fc receptor homolog 3 antibody Fc receptor-like protein 3 antibody FcR-like protein 3 antibody FcRH3 antibody FcRL3 antibody FCRL3_HUMAN antibody hIFGP3 antibody IFGP family protein 3 antibody IFGP3 antibody Immune receptor translocation-associated protein 3 antibody Immunoglobulin superfamily receptor translocation associated protein 3 antibody IRTA3 antibody SH2 domain-containing phosphatase anchor protein 2 antibody SPAP2 antibody |
|---|---|
| Molecular Weight(MW) | 80.9kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q96P31 |
|---|
Application
Western blot analysis of CD307C against human CD307C (AA: extra 18-153) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD307C against HEK293 (1) and CD307C (AA: extra 18-153)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD307C was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-77, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
