Application
Western blot analysis of CD101 against human CD101 (AA: extra 22-168) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD101 against HEK293 (1) and CD101 (AA: extra 22-168)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD101 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-81, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD101 [9A8B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CD101 (CD101 Molecule) is a Protein Coding gene. Diseases associated with CD101 include Langerhans Cell Histiocytosis and Histiocytosis. Among its related pathways are Innate Lymphoid Cell Differentiation Pathways and Innate Immune System. Gene Ontology (GO) annotations related to this gene include hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides. An important paralog of this gene is IGSF3. |
| Immunogen | Purified recombinant fragment of human CD101 (AA: extra 22-168) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | CD101 molecule antibody cell surface glycoprotein V7 antibody EWI-101 antibody glu-Trp-Ile EWI motif-containing protein 101 antibody IGSF2 antibody Immunoglobulin superfamily member 2 antibody Leukocyte surface protein antibody V7 antibody V7 LSB antibody |
|---|---|
| Molecular Weight(MW) | 115kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q93033 |
|---|
Application
Western blot analysis of CD101 against human CD101 (AA: extra 22-168) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD101 against HEK293 (1) and CD101 (AA: extra 22-168)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-81, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD101 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-81, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
