Application
Western blot analysis of CD27 against human CD27 (AA: extra 20-144) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD27 against HEK293 (1) and CD27 (AA: extra 20-144)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD27 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-82, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD27 [3B6D3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 27 antibody CD27 antibody CD27 antigen antibody CD27 molecule antibody CD27_HUMAN antibody CD27L receptor antibody LPFS2 antibody MGC20393 antibody OTTHUMP00000238557 antibody S152 antibody T cell activation antigen CD27 antibody T cell antivation antigen S152 antibody T-cell activation antigen CD27 antibody T14 antibody TNFRSF 7 antibody TNFRSF7 antibody TNFSF7 antibody Tp 55 antibody Tp55 antibody Tumor necrosis factor receptor superfamily member 7 antibody |
| Product description | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
| Immunogen | Purified recombinant fragment of human CD27 (AA: extra 20-144) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 27 antibody CD27 antibody CD27 antigen antibody CD27 molecule antibody CD27_HUMAN antibody CD27L receptor antibody LPFS2 antibody MGC20393 antibody OTTHUMP00000238557 antibody S152 antibody T cell activation antigen CD27 antibody T cell antivation antigen S152 antibody T-cell activation antigen CD27 antibody T14 antibody TNFRSF 7 antibody TNFRSF7 antibody TNFSF7 antibody Tp 55 antibody Tp55 antibody Tumor necrosis factor receptor superfamily member 7 antibody |
|---|---|
| Molecular Weight(MW) | 29.1kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P26842 |
|---|
Application
Western blot analysis of CD27 against human CD27 (AA: extra 20-144) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD27 against HEK293 (1) and CD27 (AA: extra 20-144)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD27 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-82, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
