Application
Western blot analysis of CD369 mAb against human CD369 (AA: extra 66-247) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-84, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD369 against HEK293 (1) and CD369 (AA: extra 66-247)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-84, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunocytochemistry staining of CD369 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-84, 1/100) for 1 hour at room temperature, washed with PBS. Alexa FluorApplication
Flow cytometric analysis of CD369 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-84, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD369 [4G2G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adipocyte membrane protein antibody BDPLT10 antibody CD36 antibody CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody CD36 antigen antibody CD36 molecule (thrombospondin receptor) antibody CD36 molecule antibody CD36_HUMAN antibody CHDS7 antibody Cluster determinant 36 antibody Collagen receptor, platelet antibody FAT antibody Fatty acid translocase antibody Fatty acid transport protein antibody Glycoprotein IIIb antibody GP IIIb antibody GP3B antibody GP4 antibody GPIIIB antibody GPIV antibody Leukocyte differentiation antigen CD36 antibody MGC108510 antibody MGC91634 antibody PAS 4 protein antibody PAS IV antibody PAS-4 antibody PASIV antibody Platelet collagen receptor antibody Platelet glycoprotein 4 antibody Platelet glycoprotein IV antibody scarb3 antibody Scavenger receptor class B member 3 antibody SR-B3 antibody SRB3 antibody Thrombospondin receptor antibody |
| Product description | This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. |
| Immunogen | Purified recombinant fragment of human CD369 (AA: extra 66-247) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Adipocyte membrane protein antibody
BDPLT10 antibody
CD36 antibody
CD36 antigen (collagen type I receptor thrombospondin receptor) antibody CD36 antigen antibody CD36 molecule (thrombospondin receptor) antibody CD36 molecule antibody CD36_HUMAN antibody CHDS7 antibody Cluster determinant 36 antibody Collagen receptor platelet antibody FAT antibody Fatty acid translocase antibody Fatty acid transport protein antibody Glycoprotein IIIb antibody GP IIIb antibody GP3B antibody GP4 antibody GPIIIB antibody GPIV antibody Leukocyte differentiation antigen CD36 antibody MGC108510 antibody MGC91634 antibody PAS 4 protein antibody PAS IV antibody PAS-4 antibody PASIV antibody Platelet collagen receptor antibody Platelet glycoprotein 4 antibody Platelet glycoprotein IV antibody scarb3 antibody Scavenger receptor class B member 3 antibody SR-B3 antibody SRB3 antibody Thrombospondin receptor antibody |
|---|---|
| Molecular Weight(MW) | 27.6kDa |
| Cellular Localization | Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis. |
| SwissProt ID | P16671 |
|---|
Application
Western blot analysis of CD369 mAb against human CD369 (AA: extra 66-247) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-84, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD369 against HEK293 (1) and CD369 (AA: extra 66-247)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-84, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of CD369 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1711-84, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Flow cytometric analysis of CD369 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-84, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells, Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
