Application
Western blot analysis of CD179A against human CD179A (AA: extra 20-145) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD179A against HEK293 (1) and CD179A (AA: extra 20-145)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD179A was done on MOLT4 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-87, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD179A [5F8F3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD179 antigen like family member A antibody CD179 antigen-like family member A antibody CD179a antibody IGI antibody IGVPB antibody Immunoglobulin iota chain antibody Pre B lymphocyte 1 antibody pre-B lymphocyte 1 antibody Protein VPreB1 antibody V(pre)B protein antibody VPREB 1 antibody VPREB antibody VpreB protein antibody VPREB_HUMAN antibody VPREB1 antibody |
| Product description | The protein encoded by this gene belongs to the immunoglobulin superfamily and is expressed selectively at the early stages of B cell development, namely, in proB and early preB cells. This gene encodes the iota polypeptide chain that is associated with the Ig-mu chain to form a molecular complex which is expressed on the surface of pre-B cells. The complex is thought to regulate Ig gene rearrangements in the early steps of B-cell differentiation. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD179A (AA: extra 20-145) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD179 antigen like family member A antibody CD179 antigen-like family member A antibody CD179a antibody IGI antibody IGVPB antibody Immunoglobulin iota chain antibody Pre B lymphocyte 1 antibody pre-B lymphocyte 1 antibody Protein VPreB1 antibody V(pre)B protein antibody VPREB 1 antibody VPREB antibody VpreB protein antibody VPREB_HUMAN antibody VPREB1 antibody |
|---|---|
| Molecular Weight(MW) | 16.6kDa |
| Cellular Localization | Extracellular region, extracellular space |
| SwissProt ID | P12018 |
|---|
Application
Western blot analysis of CD179A against human CD179A (AA: extra 20-145) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD179A against HEK293 (1) and CD179A (AA: extra 20-145)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD179A was done on MOLT4 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-87, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | MOLT4 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
