Application
Western blot analysis of CD368 against human CD368 (AA: extra 39-215) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD368 against HEK293 (1) and CD368 (AA: extra 39-215)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD368 was done on MOLT4 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-90, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD368 [6E4B1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 8 antibody C type lectin domain family 4, member D antibody C type lectin like receptor 6 antibody C type lectin receptor antibody C type lectin superfamily member 8 antibody C-type lectin domain family 4 member D antibody C-type lectin superfamily member 8 antibody C-type lectin-like receptor 6 antibody CLC4D_HUMAN antibody CLEC 6 antibody CLEC-6 antibody Clec4d antibody CLEC6 antibody CLECSF8 antibody lectin, C type, superfamily member 8 antibody Macrophage C type lectin antibody MCL antibody MGC40078 antibody MPCL antibody CD368 |
| Product description | This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. |
| Immunogen | Purified recombinant fragment of human CD368 (AA: extra 39-215) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 8 antibody C type lectin domain family 4 member D antibody C type lectin like receptor 6 antibody C type lectin receptor antibody C type lectin superfamily member 8 antibody C-type lectin domain family 4 member D antibody C-type lectin superfamily member 8 antibody C-type lectin-like receptor 6 antibody CLC4D_HUMAN antibody CLEC 6 antibody CLEC-6 antibody Clec4d antibody CLEC6 antibody CLECSF8 antibody lectin C type superfamily member 8 antibody Macrophage C type lectin antibody MCL antibody MGC40078 antibody MPCL antibody CD368 |
|---|---|
| Molecular Weight(MW) | 24.7kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q8WXI8 |
|---|
Application
Western blot analysis of CD368 against human CD368 (AA: extra 39-215) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD368 against HEK293 (1) and CD368 (AA: extra 39-215)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD368 was done on MOLT4 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-90, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | MOLT4 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
