Application
Western blot analysis of CD174 against human CD174 (AA: 199-361) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD174 against HEK293 (1) and CD174 (AA: 199-361)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of EDA2R was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-92, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD174 [1F8G5C7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-(1,3/1,4)-fucosyltransferase antibody Blood group Lewis alpha-4-fucosyltransferase antibody CD174 antibody FT3B antibody Fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group) antibody Fucosyltransferase 3 antibody Fucosyltransferase III antibody FucT III antibody FucT-III antibody FUT3 antibody FUT3_HUMAN antibody Galactoside 3(4)-L-fucosyltransferase antibody LE antibody Les antibody Lewis FT antibody |
| Product description | The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. |
| Immunogen | Purified recombinant fragment of human CD174 (AA: 199-361) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Alpha-(1 3/1 4)-fucosyltransferase antibody Blood group Lewis alpha-4-fucosyltransferase antibody CD174 antibody FT3B antibody Fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase Lewis blood group) antibody Fucosyltransferase 3 antibody Fucosyltransferase III antibody FucT III antibody FucT-III antibody FUT3 antibody FUT3_HUMAN antibody Galactoside 3(4)-L-fucosyltransferase antibody LE antibody Les antibody Lewis FT antibody |
|---|---|
| Molecular Weight(MW) | 42.1kDa |
| Cellular Localization | Golgi stack membrane |
| SwissProt ID | P21217 |
|---|
Application
Western blot analysis of CD174 against human CD174 (AA: 199-361) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD174 against HEK293 (1) and CD174 (AA: 199-361)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of EDA2R was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-92, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
