Application
Western blot analysis of NFKB2 against human NFKB2 (AA: 712-900) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of NFKB2 against HEK293 (1) and NFKB2 (AA: 712-900)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of NFKB2 against NIH/3T3 (1), MCF-7 (2), and THP-1 (3) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | NFKB2 [5E6A12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human NFKB2 (AA: 712-900) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | CVID10 antibody DNA binding factor KBF2 antibody H2TF1 antibody Lymphocyte translocation chromosome 10 protein antibody LYT 10 antibody NF kB2 antibody NFKB p52/p100 subunit antibody Nuclear factor Kappa B subunit 2 antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody Oncogene Lyt 10 antibody p100 antibody Transcription factor NFKB2 antibody |
|---|---|
| Molecular Weight(MW) | 96.7kDa |
| Cellular Localization | Cytoplasmic and Nuclear. |
| SwissProt ID | Q00653 |
|---|
Application
Western blot analysis of NFKB2 against human NFKB2 (AA: 712-900) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of NFKB2 against HEK293 (1) and NFKB2 (AA: 712-900)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of NFKB2 against NIH/3T3 (1), MCF-7 (2), and THP-1 (3) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | NIH/3T3, MCF-7, and THP-1 cell lysate |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
