Application
Western blot analysis of CD307A against human CD307A (AA: extra 17-202) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD307A against HEK293 (1) and CD307A (AA: extra 17-202)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD307A was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-96, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD307A [4E9B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD307A (AA: extra 17-202) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | CD307a antibody
Fc receptor homolog 1 antibody
Fc receptor like protein 1 antibody
Fc receptor-like protein 1 antibody
FcR like protein 1 antibody
FcR-like protein 1 antibody
FCRH 1 antibody
FcRH1 antibody
FCRL 1 antibody
FcRL1 antibody
FCRL1_HUMAN antibody
hIFGP 1 antibody
hIFGP1 antibody
IFGP 1 antibody
IFGP family protein 1 antibody
IFGP1 antibody
Immune receptor translocation associated protein 5 antibody
Immune receptor translocation-associated protein 5 antibody
immunoglobulin superfamily Fc receptor gp42 antibody IRTA 5 antibody IRTA5 antibody RP11 367J7.7 antibody |
|---|---|
| Molecular Weight(MW) | 47kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q96LA6 |
|---|
Application
Western blot analysis of CD307A against human CD307A (AA: extra 17-202) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD307A against HEK293 (1) and CD307A (AA: extra 17-202)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD307A was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-96, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
