Application
Western blot analysis of CD113 against human CD113 (AA: extra 282-404) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD113 against HEK293 (1) and CD113 (AA: extra 282-404)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD113 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-98, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD113 [6D5B2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD113 antibody CDw113 antibody DKFZP566B0846 antibody FLJ90624 antibody Nectin-3 antibody Nectin3 antibody Poliovirus receptor related 3 antibody Poliovirus receptor related protein 3 antibody Poliovirus receptor-like 3 antibody Poliovirus receptor-related protein 3 antibody PPR3 antibody PRR3 antibody Pvrl3 antibody PVRL3_HUMAN antibody PVRR3 antibody |
| Product description | This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human CD113 (AA: extra 282-404) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD113 antibody CDw113 antibody DKFZP566B0846 antibody FLJ90624 antibody Nectin-3 antibody Nectin3 antibody Poliovirus receptor related 3 antibody Poliovirus receptor related protein 3 antibody Poliovirus receptor-like 3 antibody Poliovirus receptor-related protein 3 antibody PPR3 antibody PRR3 antibody Pvrl3 antibody PVRL3_HUMAN antibody PVRR3 antibody |
|---|---|
| Molecular Weight(MW) | 61kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q9NQS3 |
|---|
Application
Western blot analysis of CD113 against human CD113 (AA: extra 282-404) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD113 against HEK293 (1) and CD113 (AA: extra 282-404)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD113 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-98, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
