Application
Western blot analysis of EZH1 against human EZH1 (AA: 135-294) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EZH1 against HEK293 (1) and EZH1 (AA: 135-294)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of EZH1 against HepG2 (1) and Hela (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of EZH1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-99, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | EZH1 [3B6B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Enhancer of zeste homolog 1 (Drosophila) antibody Enhancer of zeste homolog 1 antibody ENX-2 antibody ENX2 antibody Ezh1 antibody EZH1_HUMAN antibody Histone-lysine N-methyltransferase EZH1 antibody |
| Product description | The polycomb group (PcG) proteins are involved in maintaining the silenced state of multiple developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis. Enhancer of zest homolog 1 (Ezh1), is a member of this large protein family and a subunit of the polycomb repressor complex 2 (PRC2), also containing SUZ12 and EED. Ezh1 and its paralog Ezh2 are mutually exclusive catalytic subunits of the PRC2 complex, which functions to mono-, di-, and tri-methylated Lys27 on histone H3, a mark that is associated with transcriptional repression. While EZH1 is less abundant than EZH2, it is still required for cell identity and self-renewal of embryonic stem cells. Ezh1 is also required for hematopoietic stem cell maintenance and functions to prevent a senescence-like cell cycle arrest. Ezh1 is required for myogenic differentiation and hepatocyte homeostasis and regeneration. While many studies have implicated Ezh2 in multiple types of cancer, a potential role for Ezh1 is less understood. However, several studies have shown dual inhibitors of Ezh1/Ezh2 to be more effective than Ezh2-specific inhibitors in treatment of multiple myeloma, prostate cancer, diffuse large B-cell lymphoma, and leukemia, suggesting an important role for Ezh1 in cancer. |
| Immunogen | Purified recombinant fragment of human EZH1 (AA: 135-294) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Enhancer of zeste homolog 1 (Drosophila) antibody Enhancer of zeste homolog 1 antibody ENX-2 antibody ENX2 antibody Ezh1 antibody EZH1_HUMAN antibody Histone-lysine N-methyltransferase EZH1 antibody |
|---|---|
| Molecular Weight(MW) | 85.3kDa |
| Cellular Localization | Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3. |
| SwissProt ID | Q92800 |
|---|
Application
Western blot analysis of EZH1 against human EZH1 (AA: 135-294) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EZH1 against HEK293 (1) and EZH1 (AA: 135-294)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of EZH1 against HepG2 (1) and Hela (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of EZH1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1711-99, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HepG2 and Hela cell lysate, Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
