Application
Western blot analysis of CD30 against human CD30 (AA: extra 19-379) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD30 against HEK293 (1) and CD30 (AA: extra 19-379)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD30 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-04, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD30 [4D1C2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 30 antibody CD30 antibody CD30 antigen antibody CD30L receptor antibody Cytokine receptor CD30 antibody D1S166E antibody KI 1 antibody KI 1 antigen antibody Ki-1 antigen antibody KI1 antibody Lymphocyte activation antigen CD30 antibody TNFRSF 8 antibody Tnfrsf8 antibody TNR8_HUMAN antibody Tumor necrosis factor receptor superfamily member 8 antibody |
| Product description | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Immunogen | Purified recombinant fragment of human CD30 (AA: extra 19-379) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 30 antibody CD30 antibody CD30 antigen antibody CD30L receptor antibody Cytokine receptor CD30 antibody D1S166E antibody KI 1 antibody KI 1 antigen antibody Ki-1 antigen antibody KI1 antibody Lymphocyte activation antigen CD30 antibody TNFRSF 8 antibody Tnfrsf8 antibody TNR8_HUMAN antibody Tumor necrosis factor receptor superfamily member 8 antibody |
|---|---|
| Molecular Weight(MW) | 63.7kDa |
| Cellular Localization | Cytoplasm and Cell membrane. |
| SwissProt ID | P28908 |
|---|
Application
Western blot analysis of CD30 against human CD30 (AA: extra 19-379) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD30 against HEK293 (1) and CD30 (AA: extra 19-379)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD30 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-04, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
