Application
Western blot analysis of KMT2C against human KMT2C (AA: 1-205) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of KMT2C against HEK293 (1) and KMT2C (AA: 1-205)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunocytochemistry staining of KMT2C in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1712-05, 1/100) for 1 hour at room temperature, washed with PBS. Alexa FluorApplication
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-KMT2C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-05, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Flow cytometric analysis of KMT2C was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-05, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | KMT2C [6D1B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a nuclear protein with an AT hook DNA-binding domain, a DHHC-type zinc finger, six PHD-type zinc fingers, a SET domain, a post-SET domain and a RING-type zinc finger. This protein is a member of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. |
| Immunogen | Purified recombinant fragment of human KMT2C (AA: 1-205) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,IHC,ICC,FC |
| WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | Histone-lysine N-methyltransferase MLL3 antibody Homologous to ALR protein antibody KMT2C antibody Lysine N-methyltransferase 2C antibody MLL3 antibody MLL3_HUMAN antibody Myeloid/lymphoid or mixed-lineage leukemia protein 3 antibody Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog antibody |
|---|---|
| Molecular Weight(MW) | 541.4kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q8NEZ4 |
|---|
Application
Western blot analysis of KMT2C against human KMT2C (AA: 1-205) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of KMT2C against HEK293 (1) and KMT2C (AA: 1-205)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of KMT2C in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1712-05, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-KMT2C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-05, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of KMT2C was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-05, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells, cervical cancer tissues |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
