Application
Western blot analysis of CD181 against human CD181 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD181 against HEK293 (1) and CD181-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | CD181 [2B8A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C C antibody C C CKR 1 antibody C-X-C chemokine receptor type 1 antibody CCCKR1 antibody CD 181 antibody CD128 antibody CD181 antibody CD181 antigen antibody CDw128a antibody Chemokine (C X C motif) receptor 1 antibody Chemokine (C X C) receptor 1 antibody Chemokine receptor 1 antibody CKR 1 antibody CMKAR1 antibody CXC-R1 antibody CXCR 1 antibody CXCR-1 antibody CXCR1 antibody CXCR1_HUMAN antibody High affinity interleukin 8 receptor A antibody High affinity interleukin-8 receptor A antibody IL 8 receptor type 1 antibody IL 8R A antibody IL-8 receptor type 1 antibody IL-8R A antibody IL8 receptor antibody IL8 receptor type 1 antibody IL8R1 antibody IL8RA antibody IL8RBA antibody Interleukin 8 receptor alpha antibody Interleukin 8 receptor type 1 antibody Interleukin 8 receptor type A antibody |
| Product description | The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. |
| Immunogen | Purified recombinant fragment of human CD181 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | C C antibody C C CKR 1 antibody C-X-C chemokine receptor type 1 antibody CCCKR1 antibody CD 181 antibody CD128 antibody CD181 antibody CD181 antigen antibody CDw128a antibody Chemokine (C X C motif) receptor 1 antibody Chemokine (C X C) receptor 1 antibody Chemokine receptor 1 antibody CKR 1 antibody CMKAR1 antibody CXC-R1 antibody CXCR 1 antibody CXCR-1 antibody CXCR1 antibody CXCR1_HUMAN antibody High affinity interleukin 8 receptor A antibody High affinity interleukin-8 receptor A antibody IL 8 receptor type 1 antibody IL 8R A antibody IL-8 receptor type 1 antibody IL-8R A antibody IL8 receptor antibody IL8 receptor type 1 antibody IL8R1 antibody IL8RA antibody IL8RBA antibody Interleukin 8 receptor alpha antibody Interleukin 8 receptor type 1 antibody Interleukin 8 receptor type A antibody |
|---|---|
| Molecular Weight(MW) | 39.8kDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | P25024 |
|---|
Application
Western blot analysis of CD181 against human CD181 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD181 against HEK293 (1) and CD181-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-06, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Highest expression level in blood. |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
