Application
Western blot analysis of CD301 against human CD301 (AA: extra 158-316) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD301 against HEK293 (1) and CD301 (AA: extra 158-316)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD301 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-08, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD301 [2C5C6F10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| Immunogen | Purified recombinant fragment of human CD301 (AA: extra 158-316) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 13 (macrophage derived) antibody
C type (calcium dependent carbohydrate recognition domain) lectin superfamily member 14 (macrophage derived) antibody
C type lectin domain family 10 member A antibody C type lectin superfamily member 14 antibody C-type lectin domain family 10 member A antibody C-type lectin superfamily member 14 antibody CD301 antibody CD301 antigen antibody CD301A antibody CLC10_HUMAN antibody CLEC10A antibody CLECSF13 antibody CLECSF14 antibody HML antibody HML2 antibody Lectin antibody Macrophage C type lectin antibody MACROPHAGE GALACTOSE-TYPE C-TYPE antibody Macrophage lectin 2 (calcium dependent) antibody Macrophage lectin 2 antibody MGL antibody Mgl1 antibody |
|---|---|
| Molecular Weight(MW) | 35.4kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q8IUN9 |
|---|
Application
Western blot analysis of CD301 against human CD301 (AA: extra 158-316) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD301 against HEK293 (1) and CD301 (AA: extra 158-316)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-08, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD301 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-08, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
