Application
Western blot analysis of KMT2A against human KMT2A (AA: 801-956) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of KMT2A against HEK293 (1) and KMT2A (AA: 801-956)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | KMT2A [4A5A12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human KMT2A (AA: 801-956) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | ALL-1 antibody ALL1 antibody C-terminal cleavage product of 180 kDa antibody CXXC-type zinc finger protein 7 antibody CXXC7 antibody HRX antibody HTRX1 antibody KMT2A antibody Lysine N-methyltransferase 2A antibody Mll antibody MLL cleavage product C180 antibody MLL1 antibody MLL1_HUMAN antibody MLL1A antibody N-terminal cleavage product of 320 kDa antibody p180 antibody p320 antibody Trithorax-like protein antibody TRX1 antibody Zinc finger protein HRX antibody |
|---|---|
| Molecular Weight(MW) | 431.7kDa |
| Cellular Localization | Nucleus and Nucleus. Localizes to a diffuse nuclear pattern when not associated with MLL cleavage product N320. |
| SwissProt ID | Q03164 |
|---|
Application
Western blot analysis of KMT2A against human KMT2A (AA: 801-956) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of KMT2A against HEK293 (1) and KMT2A (AA: 801-956)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Heart, lung, brain and T- and B-lymphocytes. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
