Application
Western blot analysis of SETD7 against human SETD7 (AA: 107-366) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of SETD7 against HEK293 (1) and SETD7 (AA: 107-366)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of SETD7 against MCF-7 (1), Hela (2), A549 (3), COS7 (4), Jurkat (5), and PC-12 (6) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of SETD7 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-11, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | SETD7 [1B3B8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ21193 antibody H3 K4 HMTase antibody H3-K4-HMTase SETD7 antibody H4 lysine 4 specific antibody Histone H3 K4 methyltransferase antibody Histone H3 lysine 4 specific methyltransferase antibody Histone H3-K4 methyltransferase SETD7 antibody Histone H4 K4 methyltransferase antibody Histone lysine N methyltransferase antibody Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody Histone-lysine N-methyltransferase SETD7 antibody KIAA1717 antibody KMT7 antibody Lysine methyltransferase antibody Lysine N-methyltransferase 7 antibody OTTHUMP00000164543 antibody OTTHUMP00000220049 antibody SET 7 antibody SET 7/9 antibody SET 9 antibody SET D7 antibody SET domain containing (lysine methyltransferase) 7 antibody SET domain containing protein 7 antibody SET domain containing protein 8 antibody SET domain-containing protein 7 antibody SET7 antibody SET7/9 antibody SET9 antibody Setd7 antibody SETD7_HUMAN antibody |
| Product description | Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates Lys-189 of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates Lys-372 of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. |
| Immunogen | Purified recombinant fragment of human SETD7 (AA: 107-366) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | HumanRatMonkey |
| Concentration | 1mg/mL |
| Alternative Names | FLJ21193 antibody H3 K4 HMTase antibody H3-K4-HMTase SETD7 antibody H4 lysine 4 specific antibody Histone H3 K4 methyltransferase antibody Histone H3 lysine 4 specific methyltransferase antibody Histone H3-K4 methyltransferase SETD7 antibody Histone H4 K4 methyltransferase antibody Histone lysine N methyltransferase antibody Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody Histone-lysine N-methyltransferase SETD7 antibody KIAA1717 antibody KMT7 antibody Lysine methyltransferase antibody Lysine N-methyltransferase 7 antibody OTTHUMP00000164543 antibody OTTHUMP00000220049 antibody SET 7 antibody SET 7/9 antibody SET 9 antibody SET D7 antibody SET domain containing (lysine methyltransferase) 7 antibody SET domain containing protein 7 antibody SET domain containing protein 8 antibody SET domain-containing protein 7 antibody SET7 antibody SET7/9 antibody SET9 antibody Setd7 antibody SETD7_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 40.7kDa |
| Cellular Localization | Nucleus. Chromosome. |
| SwissProt ID | Q8WTS6 |
|---|
Application
Western blot analysis of SETD7 against human SETD7 (AA: 107-366) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of SETD7 against HEK293 (1) and SETD7 (AA: 107-366)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of SETD7 against MCF-7 (1), Hela (2), A549 (3), COS7 (4), Jurkat (5), and PC-12 (6) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of SETD7 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-11, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | MCF-7, Hela, A549, COS7, Jurkat, and PC-12 cell lysate, Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
