Application
Western blot analysis of ACSS1 against human ACSS1 (AA: 548-689) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of ACSS1 against HEK293 (1) and ACSS1 (AA: 548-689)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-ACSS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-12, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Flow cytometric analysis of ACSS1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-12, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | ACSS1 [3A7C1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACAS2L antibody AceCS2 antibody AceCS2L antibody Acetate CoA ligase 2 antibody Acetate--CoA ligase 2 antibody Acetyl CoA synthetase 2 antibody Acetyl Coenzyme A synthetase 2 (AMP forming) like antibody Acetyl coenzyme A synthetase 2 like, mitochondrial antibody Acetyl-CoA synthetase 2 antibody Acetyl-coenzyme A synthetase 2-like, mitochondrial antibody ACS2L_HUMAN antibody Acss1 antibody Acyl CoA synthetase short chain family member 1 antibody Acyl-CoA synthetase short-chain family member 1 antibody FLJ45659 antibody KIAA1846 antibody MGC33843 antibody |
| Product description | This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Immunogen | Purified recombinant fragment of human ACSS1 (AA: 548-689) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | ACAS2L antibody
AceCS2 antibody
AceCS2L antibody
Acetate CoA ligase 2 antibody
Acetate--CoA ligase 2 antibody
Acetyl CoA synthetase 2 antibody
Acetyl Coenzyme A synthetase 2 (AMP forming) like antibody
Acetyl coenzyme A synthetase 2 like mitochondrial antibody Acetyl-CoA synthetase 2 antibody Acetyl-coenzyme A synthetase 2-like mitochondrial antibody ACS2L_HUMAN antibody Acss1 antibody Acyl CoA synthetase short chain family member 1 antibody Acyl-CoA synthetase short-chain family member 1 antibody FLJ45659 antibody KIAA1846 antibody MGC33843 antibody |
|---|---|
| Molecular Weight(MW) | 74.8kDa |
| Cellular Localization | Mitochondrion matrix. |
| SwissProt ID | Q9NUB1 |
|---|
Application
Western blot analysis of ACSS1 against human ACSS1 (AA: 548-689) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of ACSS1 against HEK293 (1) and ACSS1 (AA: 548-689)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-ACSS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-12, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of ACSS1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-12, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells, cervical cancer tissues |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
