Application
Western blot analysis of CD218A against human CD218A (AA: extra 22-185) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD218A against HEK293 (1) and CD218A (AA: extra 22-185)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD218A was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-15, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD218A [2B7E6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD218 antigen-like family member A antibody CD218a antibody CDw218a antibody CDw218a antigen antibody IL 1Rrp antibody IL-18R-1 antibody IL-18R1 antibody IL-1Rrp antibody IL1 receptor related protein antibody IL1 receptor-related protein antibody IL18R_HUMAN antibody IL18R1 antibody IL18RA antibody Il18ralpha antibody IL1R-rp antibody IL1RRP antibody Interleukin 18 receptor 1 antibody Interleukin 18 receptor, alpha chain antibody Interleukin-18 receptor 1 antibody |
| Product description | The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human CD218A (AA: extra 22-185) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD218 antigen-like family member A antibody
CD218a antibody
CDw218a antibody
CDw218a antigen antibody
IL 1Rrp antibody
IL-18R-1 antibody
IL-18R1 antibody
IL-1Rrp antibody
IL1 receptor related protein antibody
IL1 receptor-related protein antibody
IL18R_HUMAN antibody
IL18R1 antibody
IL18RA antibody
Il18ralpha antibody
IL1R-rp antibody
IL1RRP antibody
Interleukin 18 receptor 1 antibody
Interleukin 18 receptor alpha chain antibody Interleukin-18 receptor 1 antibody |
|---|---|
| Molecular Weight(MW) | 62.3kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q13478 |
|---|
Application
Western blot analysis of CD218A against human CD218A (AA: extra 22-185) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD218A against HEK293 (1) and CD218A (AA: extra 22-185)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD218A was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-15, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
