Application
Western blot analysis of NR2C2 against human NR2C2 (AA: 62-356) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of NR2C2 against HEK293 (1) and NR2C2 (AA: 62-356)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of NR2C2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-16, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | NR2C2 [8F2E8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hTAK1 antibody Nr2c2 antibody NR2C2_HUMAN antibody Nuclear hormone receptor TR4 antibody Nuclear receptor subfamily 2 group C member 2 antibody Orphan nuclear receptor TAK1 antibody Orphan nuclear receptor TR4 antibody TAK1 antibody Testicular nuclear receptor 4 antibody Testicular receptor 4 antibody TR2R1 antibody TR4 antibody TR4 nuclear hormone receptor antibody |
| Product description | This gene encodes a protein that belongs to the nuclear hormone receptor family. Members of this family act as ligand-activated transcription factors and function in many biological processes such as development, cellular differentiation and homeostasis. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes. The protein encoded by this gene plays a role in protecting cells from oxidative stress and damage induced by ionizing radiation. The lack of a similar gene in mouse results in growth retardation, severe spinal curvature, subfertility, premature aging, and prostatic intraepithelial neoplasia (PIN) development. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen | Purified recombinant fragment of human NR2C2 (AA: 62-356) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | hTAK1 antibody Nr2c2 antibody NR2C2_HUMAN antibody Nuclear hormone receptor TR4 antibody Nuclear receptor subfamily 2 group C member 2 antibody Orphan nuclear receptor TAK1 antibody Orphan nuclear receptor TR4 antibody TAK1 antibody Testicular nuclear receptor 4 antibody Testicular receptor 4 antibody TR2R1 antibody TR4 antibody TR4 nuclear hormone receptor antibody |
|---|---|
| Molecular Weight(MW) | 65.4kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P49116 |
|---|
Application
Western blot analysis of NR2C2 against human NR2C2 (AA: 62-356) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of NR2C2 against HEK293 (1) and NR2C2 (AA: 62-356)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of NR2C2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-16, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
