Application
Western blot analysis of RBBP7 against human RBBP7 (AA: 1-200) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of RBBP7 against HEK293 (1) and RBBP7 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of RBBP7 against Jurkat (1), HepG2 (2), F9 (3), C6 (4), LNCAP (5), HL-60 (6), Hela (7), and SH-SY5Y (8) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunocytochemistry staining of RBBP7 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1712-17, 1/100) for 1 hour at room temperature, washed with PBS. Alexa FluorApplication
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using anti-RBBP7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-17, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using anti-RBBP7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-17, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Flow cytometric analysis of RBBP7 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-17, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | RBBP7 [2A10C2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G1/S transition control protein binding protein RbAp46 antibody Histone acetyltransferase type B subunit 2 antibody Histone binding protein RBBP7 antibody Histone-binding protein RBBP7 antibody MGC138867 antibody MGC138868 antibody Nucleosome remodeling factor subunit RBAP46 antibody Nucleosome-remodeling factor subunit RBAP46 antibody RBAP46 antibody RBBP 7 antibody RBBP-7 antibody RBBP7 antibody RBBP7_HUMAN antibody Retinoblastoma binding protein 7 antibody Retinoblastoma binding protein p46 antibody Retinoblastoma-binding protein 7 antibody Retinoblastoma-binding protein p46 antibody Retinoblastoma-binding protein RbAp46 antibody |
| Product description | This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human RBBP7 (AA: 1-200) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | G1/S transition control protein binding protein RbAp46 antibody Histone acetyltransferase type B subunit 2 antibody Histone binding protein RBBP7 antibody Histone-binding protein RBBP7 antibody MGC138867 antibody MGC138868 antibody Nucleosome remodeling factor subunit RBAP46 antibody Nucleosome-remodeling factor subunit RBAP46 antibody RBAP46 antibody RBBP 7 antibody RBBP-7 antibody RBBP7 antibody RBBP7_HUMAN antibody Retinoblastoma binding protein 7 antibody Retinoblastoma binding protein p46 antibody Retinoblastoma-binding protein 7 antibody Retinoblastoma-binding protein p46 antibody Retinoblastoma-binding protein RbAp46 antibody |
|---|---|
| Molecular Weight(MW) | 47.8kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q16576 |
|---|
Application
Western blot analysis of RBBP7 against human RBBP7 (AA: 1-200) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of RBBP7 against HEK293 (1) and RBBP7 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of RBBP7 against Jurkat (1), HepG2 (2), F9 (3), C6 (4), LNCAP (5), HL-60 (6), Hela (7), and SH-SY5Y (8) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunocytochemistry staining of RBBP7 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1712-17, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor
Application
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using anti-RBBP7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-17, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using anti-RBBP7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-17, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of RBBP7 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-17, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Jurkat, HepG2, F9, C6, LNCAP, HL-60, Hela, and SH-SY5Y cell lysate, Hela cells, bladder cancer tissues, rectum cancer tissues |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1：50-1:200 ICC:1：50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
