Application
Western blot analysis of LILRB1 against human LILRB1 (AA: extra 338-461) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of LILRB1 against HEK293 (1) and LILRB1 (AA: extra 338-461)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | LILRB1 [2G5B5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD85 antibody CD85 antigen antibody CD85 antigen like family member J antibody CD85 antigen-like family member J antibody CD85j antibody CD85j antigen antibody Ig like transcript 2 antibody ILT 2 antibody ILT-2 antibody ILT2 antibody immunoglobulin like transcript 2 antibody Immunoglobulin-like transcript 2 antibody leukocyte Ig like receptor 1 antibody Leukocyte immunoglobulin like receptor 1 antibody leukocyte immunoglobulin like receptor subfamily B member 1 antibody Leukocyte immunoglobulin like receptor subfamily B member 1 precursor antibody leukocyte immunoglobulin like receptor subfamily B member 1 soluble isoform antibody leukocyte immunoglobulin like receptor, subfamily B, member 1 antibody Leukocyte immunoglobulin-like receptor 1 antibody Leukocyte immunoglobulin-like receptor subfamily B member 1 antibody leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform antibody leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1 antibody LILRB1 antibody LIR 1 antibody LIR-1 antibody LIR1 antibody LIRB1_HUMAN antibody MIR 7 antibody MIR-7 antibody MIR7 antibody Monocyte/macrophage immunoglobulin like receptor 7 antibody Monocyte/macrophage immunoglobulin-like receptor 7 antibody PIR B antibody PIRB antibody |
| Product description | This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Purified recombinant fragment of human LILRB1 (AA: extra 338-461) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD85 antibody
CD85 antigen antibody
CD85 antigen like family member J antibody
CD85 antigen-like family member J antibody
CD85j antibody
CD85j antigen antibody
Ig like transcript 2 antibody
ILT 2 antibody
ILT-2 antibody
ILT2 antibody
immunoglobulin like transcript 2 antibody
Immunoglobulin-like transcript 2 antibody
leukocyte Ig like receptor 1 antibody
Leukocyte immunoglobulin like receptor 1 antibody
leukocyte immunoglobulin like receptor subfamily B member 1 antibody
Leukocyte immunoglobulin like receptor subfamily B member 1 precursor antibody
leukocyte immunoglobulin like receptor subfamily B member 1 soluble isoform antibody
leukocyte immunoglobulin like receptor subfamily B member 1 antibody Leukocyte immunoglobulin-like receptor 1 antibody Leukocyte immunoglobulin-like receptor subfamily B member 1 antibody leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform antibody leukocyte immunoglobulin-like receptor subfamily B (with TM and ITIM domains) member 1 antibody LILRB1 antibody LIR 1 antibody LIR-1 antibody LIR1 antibody LIRB1_HUMAN antibody MIR 7 antibody MIR-7 antibody MIR7 antibody Monocyte/macrophage immunoglobulin like receptor 7 antibody Monocyte/macrophage immunoglobulin-like receptor 7 antibody PIR B antibody PIRB antibody |
|---|---|
| Molecular Weight(MW) | 70.8kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q8NHL6 |
|---|
Application
Western blot analysis of LILRB1 against human LILRB1 (AA: extra 338-461) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of LILRB1 against HEK293 (1) and LILRB1 (AA: extra 338-461)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Expressed in B cells, monocytes and various dendritic cell (DC) subsets including myeloid, plasmacytoid and tolerogenic DCs (at protein level) |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
