Application
Western blot analysis of KDM2A against human KDM2A (AA: 1-150) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of KDM2A against HEK293 (1) and KDM2A (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | KDM2A [5C11F7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | [Histone-H3]-lysine-36 demethylase 1A antibody CXXC-type zinc finger protein 8 antibody CXXC8 antibody F box / LRR repeat protein 11 antibody F box and leucine rich repeat protein 11 antibody F box protein FBL7 antibody F-box and leucine-rich repeat protein 11 antibody F-box protein FBL7 antibody F-box protein Lilina antibody F-box/LRR-repeat protein 11 antibody FBL11 antibody FBL7 antibody FBXL11 antibody JHDM1A antibody JmjC domain-containing histone demethylation protein 1A antibody Jumonji C domain containing histone demethylase 1A antibody kdm2a antibody KDM2A_HUMAN antibody LILINA antibody Lysine (K) specific demethylase 2A antibody Lysine-specific demethylase 2A antibody |
| Product description | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human KDM2A (AA: 1-150) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | [Histone-H3]-lysine-36 demethylase 1A antibody CXXC-type zinc finger protein 8 antibody CXXC8 antibody F box / LRR repeat protein 11 antibody F box and leucine rich repeat protein 11 antibody F box protein FBL7 antibody F-box and leucine-rich repeat protein 11 antibody F-box protein FBL7 antibody F-box protein Lilina antibody F-box/LRR-repeat protein 11 antibody FBL11 antibody FBL7 antibody FBXL11 antibody JHDM1A antibody JmjC domain-containing histone demethylation protein 1A antibody Jumonji C domain containing histone demethylase 1A antibody kdm2a antibody KDM2A_HUMAN antibody LILINA antibody Lysine (K) specific demethylase 2A antibody Lysine-specific demethylase 2A antibody |
|---|---|
| Molecular Weight(MW) | 132.8kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9Y2K7 |
|---|
Application
Western blot analysis of KDM2A against human KDM2A (AA: 1-150) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of KDM2A against HEK293 (1) and KDM2A (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Widely expressed, with highest levels in brain, testis and ovary, followed by lung. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
