Application
Western blot analysis of XIAP against human XIAP (AA: 1-268) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of XIAP against HEK293 (1) and XIAP (AA: 1-268)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of XIAP was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-21, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | XIAP [4C12G10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP 13 antibody API3 antibody Apoptosis Inhibitor 3 antibody Baculoviral IAP repeat containing 4 antibody Baculoviral IAP Repeat Containing Protein 4 antibody Baculoviral IAP repeat-containing protein 4 antibody BIRC 4 antibody BIRC4 antibody E3 ubiquitin-protein ligase XIAP antibody hIAP-3 antibody hIAP3 antibody HILP antibody IAP 3 antibody IAP like protein antibody IAP-3 antibody IAP-like protein antibody IAP3 antibody ILP 1 antibody ILP antibody ILP1 antibody Inhibitor of apoptosis protein 3 antibody Inhibitor of Apoptosis X Linked antibody Mammalian IAP Homologue A antibody MIHA antibody X linked IAP antibody X linked inhibitor of apoptosis antibody X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody X linked inhibitor of apoptosis protein antibody X-linked IAP antibody X-linked inhibitor of apoptosis protein antibody Xiap antibody XIAP_HUMAN antibody XLP2 antibody |
| Product description | This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11. |
| Immunogen | Purified recombinant fragment of human XIAP (AA: 1-268) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | AP 13 antibody API3 antibody Apoptosis Inhibitor 3 antibody Baculoviral IAP repeat containing 4 antibody Baculoviral IAP Repeat Containing Protein 4 antibody Baculoviral IAP repeat-containing protein 4 antibody BIRC 4 antibody BIRC4 antibody E3 ubiquitin-protein ligase XIAP antibody hIAP-3 antibody hIAP3 antibody HILP antibody IAP 3 antibody IAP like protein antibody IAP-3 antibody IAP-like protein antibody IAP3 antibody ILP 1 antibody ILP antibody ILP1 antibody Inhibitor of apoptosis protein 3 antibody Inhibitor of Apoptosis X Linked antibody Mammalian IAP Homologue A antibody MIHA antibody X linked IAP antibody X linked inhibitor of apoptosis antibody X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody X linked inhibitor of apoptosis protein antibody X-linked IAP antibody X-linked inhibitor of apoptosis protein antibody Xiap antibody XIAP_HUMAN antibody XLP2 antibody |
|---|---|
| Molecular Weight(MW) | 56.7kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P98170 |
|---|
Application
Western blot analysis of XIAP against human XIAP (AA: 1-268) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of XIAP against HEK293 (1) and XIAP (AA: 1-268)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of XIAP was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-21, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
