Application
Western blot analysis of SETD2 against human SETD2 (AA: 2054-2245) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of SETD2 against HEK293 (1) and SETD2 (AA: 2054-2245)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of SETD2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-25, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | SETD2 [4G11E6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. |
| Immunogen | Purified recombinant fragment of human SETD2 (AA: 2054-2245) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,FC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | EC 2.1.1.43 antibody
FLJ16420 antibody
FLJ22472 antibody
FLJ23184 antibody
FLJ45883 antibody
HBP231 antibody
HIF 1 antibody
HIF-1 antibody
HIF1 antibody
HIP-1 antibody
Histone lysine N methyltransferase SETD2 antibody
Histone-lysine N-methyltransferase SETD2 antibody
hSET2 antibody
HSPC069 antibody
Huntingtin interacting protein 1 antibody
Huntingtin interacting protein antibody
Huntingtin interacting protein B antibody
Huntingtin interacting protein HYPB antibody
Huntingtin yeast partner B antibody
Huntingtin-binding protein 231-KD antibody Huntingtin-interacting protein 1 antibody Huntingtin-interacting protein B antibody HYPB antibody KIAA1732 antibody KMT3A antibody Lysine N methyltransferase 3A antibody Lysine N-methyltransferase 3A antibody p231HBP antibody SET domain containing 2 antibody SET domain-containing protein 2 antibody SET2 antibody SETD2 antibody SETD2_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 287.6kDa |
| Cellular Localization | Nucleus. Chromosome. |
| SwissProt ID | Q9BYW2 |
|---|
Application
Western blot analysis of SETD2 against human SETD2 (AA: 2054-2245) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of SETD2 against HEK293 (1) and SETD2 (AA: 2054-2245)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of SETD2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-25, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
