Application
Western blot analysis of CD5 against human CD5 (AA: extra 25–372) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD5 against HEK293 (1) and CD5 (AA: extra 25–372)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of CD5 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-26, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD5 [3D3G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD 5 antibody CD5 antibody CD5 antigen (p56 62) antibody CD5 antigen antibody CD5 molecule antibody CD5_HUMAN antibody LEU1 antibody LyA antibody Lymphocyte antigen T1/Leu-1 antibody OTTHUMP00000236973 antibody T-cell surface glycoprotein CD5 antibody T1 antibody |
| Product description | This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen | Purified recombinant fragment of human CD5 (AA: extra 25–372) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD 5 antibody CD5 antibody CD5 antigen (p56 62) antibody CD5 antigen antibody CD5 molecule antibody CD5_HUMAN antibody LEU1 antibody LyA antibody Lymphocyte antigen T1/Leu-1 antibody OTTHUMP00000236973 antibody T-cell surface glycoprotein CD5 antibody T1 antibody |
|---|---|
| Molecular Weight(MW) | 54.6kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P06127 |
|---|
Application
Western blot analysis of CD5 against human CD5 (AA: extra 25–372) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD5 against HEK293 (1) and CD5 (AA: extra 25–372)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of CD5 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-26, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
