Application
Western blot analysis of CD315 against human CD315 (AA: extra 46-221) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD315 against HEK293 (1) and CD315 (AA: extra 46-221)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-CD315 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-27, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Flow cytometric analysis of CD315 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-27, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | CD315 [4E9G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD315 antibody CD9 partner 1 antibody CD9P-1 antibody CD9P1 antibody EWI F antibody EWI-F antibody FLJ11001 antibody FPRP antibody FPRP_HUMAN antibody Glu Trp Ile EWI motif containing protein F antibody Glu-Trp-Ile EWI motif-containing protein F antibody KIAA1436 antibody Prostaglandin F2 alpha receptor associated protein antibody Prostaglandin F2 alpha receptor regulatory protein antibody Prostaglandin F2 receptor inhibitor antibody Prostaglandin F2 receptor negative regulator antibody Prostaglandin F2-alpha receptor regulatory protein antibody Prostaglandin F2-alpha receptor-associated protein antibody Ptgfrn antibody SMAP 6 antibody |
| Product description | Inhibits the binding of prostaglandin F2-alpha (PGF2-alpha) to its specific FP receptor, by decreasing the receptor number rather than the affinity constant. Functional coupling with the prostaglandin F2-alpha receptor seems to occur (By similarity). |
| Immunogen | Purified recombinant fragment of human CD315 (AA: extra 46-221) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD315 antibody CD9 partner 1 antibody CD9P-1 antibody CD9P1 antibody EWI F antibody EWI-F antibody FLJ11001 antibody FPRP antibody FPRP_HUMAN antibody Glu Trp Ile EWI motif containing protein F antibody Glu-Trp-Ile EWI motif-containing protein F antibody KIAA1436 antibody Prostaglandin F2 alpha receptor associated protein antibody Prostaglandin F2 alpha receptor regulatory protein antibody Prostaglandin F2 receptor inhibitor antibody Prostaglandin F2 receptor negative regulator antibody Prostaglandin F2-alpha receptor regulatory protein antibody Prostaglandin F2-alpha receptor-associated protein antibody Ptgfrn antibody SMAP 6 antibody |
|---|---|
| Molecular Weight(MW) | 98.6kDa |
| Cellular Localization | Endoplasmic reticulum membrane. Golgi apparatus. |
| SwissProt ID | Q9P2B2 |
|---|
Application
Western blot analysis of CD315 against human CD315 (AA: extra 46-221) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD315 against HEK293 (1) and CD315 (AA: extra 46-221)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using anti-CD315 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1712-27, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Flow cytometric analysis of CD315 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-27, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | HL-60 cells, cervical cancer tissues |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
