Application
Western blot analysis of CD224 against human CD224 (AA: extra 381-470) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of CD224 against HEK293 (1) and CD224 (AA: extra 381-470)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | CD224 [1F1B5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD224 antibody D22S672 antibody D22S732 antibody Gamma glutamyl transpeptidase antibody Gamma glutamyltransferase 1 antibody Gamma glutamyltranspeptidase 1 antibody Gamma-glutamyltransferase 1 antibody Gamma-glutamyltranspeptidase 1 light chain antibody GGT 1 antibody GGT antibody GGT1 antibody GGT1_HUMAN antibody Glutamyl transpeptidase antibody Glutathione hydrolase 1 antibody GTG antibody Leukotriene C4 hydrolase antibody MGC96892 antibody MGC96904 antibody MGC96963 antibody OTTHUMP00000028921 antibody OTTHUMP00000197959 antibody |
| Product description | The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. |
| Immunogen | Purified recombinant fragment of human CD224 (AA: extra 381-470) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CD224 antibody D22S672 antibody D22S732 antibody Gamma glutamyl transpeptidase antibody Gamma glutamyltransferase 1 antibody Gamma glutamyltranspeptidase 1 antibody Gamma-glutamyltransferase 1 antibody Gamma-glutamyltranspeptidase 1 light chain antibody GGT 1 antibody GGT antibody GGT1 antibody GGT1_HUMAN antibody Glutamyl transpeptidase antibody Glutathione hydrolase 1 antibody GTG antibody Leukotriene C4 hydrolase antibody MGC96892 antibody MGC96904 antibody MGC96963 antibody OTTHUMP00000028921 antibody OTTHUMP00000197959 antibody |
|---|---|
| Molecular Weight(MW) | 61.4kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P19440 |
|---|
Application
Western blot analysis of CD224 against human CD224 (AA: extra 381-470) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of CD224 against HEK293 (1) and CD224 (AA: extra 381-470)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
