Application
Western blot analysis of BNIP3L against human BNIP3L (AA: 1-187) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of BNIP3L against HEK293 (1) and BNIP3L (AA: 1-187)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | BNIP3L [1H10B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenovirus E1B19k binding protein B5 antibody Adenovirus E1B19K-binding protein B5 antibody BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody BNI3L_HUMAN antibody BNIP3a antibody BNIP3H antibody BNIP3L antibody BNIP3L protein antibody NIP3 like protein X antibody NIP3-like protein X antibody NIP3L antibody NIX antibody |
| Product description | Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3alpha, and Nix (for Nip3-like protein X). Bnip3L/Bnip3alpha/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins. |
| Immunogen | Purified recombinant fragment of human BNIP3L (AA: 1-187) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Adenovirus E1B19k binding protein B5 antibody Adenovirus E1B19K-binding protein B5 antibody BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody BNI3L_HUMAN antibody BNIP3a antibody BNIP3H antibody BNIP3L antibody BNIP3L protein antibody NIP3 like protein X antibody NIP3-like protein X antibody NIP3L antibody NIX antibody |
|---|---|
| Molecular Weight(MW) | 23.9kDa |
| Cellular Localization | Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane. |
| SwissProt ID | O60238 |
|---|
Application
Western blot analysis of BNIP3L against human BNIP3L (AA: 1-187) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of BNIP3L against HEK293 (1) and BNIP3L (AA: 1-187)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | Highest expression level in blood. |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
