Application
Western blot analysis of ATR against human ATR (AA: 366-550) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of ATR against HEK293 (1) and ATR (AA: 366-550)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of ATR was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-31, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | ATR [3G6D2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ataxia telangiectasia and Rad3 related antibody Ataxia telangiectasia and Rad3-related protein antibody ATR antibody ATR_HUMAN antibody FCTCS antibody FRAP Related Protein 1 antibody FRAP-related protein 1 antibody FRP1 antibody MEC1 antibody MEC1 mitosis entry checkpoint 1 homolog antibody Protein kinase ATR antibody RAC3 antibody Rad3 related protein antibody SCKL antibody SCKL1 antibody Serine/threonine protein kinase ATR antibody Serine/threonine-protein kinase ATR antibody |
| Product description | The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1. |
| Immunogen | Purified recombinant fragment of human ATR (AA: 366-550) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Ataxia telangiectasia and Rad3 related antibody Ataxia telangiectasia and Rad3-related protein antibody ATR antibody ATR_HUMAN antibody FCTCS antibody FRAP Related Protein 1 antibody FRAP-related protein 1 antibody FRP1 antibody MEC1 antibody MEC1 mitosis entry checkpoint 1 homolog antibody Protein kinase ATR antibody RAC3 antibody Rad3 related protein antibody SCKL antibody SCKL1 antibody Serine/threonine protein kinase ATR antibody Serine/threonine-protein kinase ATR antibody |
|---|---|
| Molecular Weight(MW) | 301kDa |
| Cellular Localization | Nucleus. Nucleus. |
| SwissProt ID | Q13535 |
|---|
Application
Western blot analysis of ATR against human ATR (AA: 366-550) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of ATR against HEK293 (1) and ATR (AA: 366-550)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of ATR was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-31, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
