Application
Western blot analysis of ATG10 against human ATG10 (AA: 1-125) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of ATG10 against HEK293 (1) and ATG10 (AA: 1-125)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of ATG10 against K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of ATG10 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-38, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | ATG10 [8A9B3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apg 10 antibody APG 10L antibody APG10 antibody APG10 autophagy 10 like antibody APG10 like antibody APG10, S. cerevisiae, homolog of antibody APG10-like antibody APG10L antibody ATG 10 antibody ATG10 antibody ATG10 autophagy related 10 homolog (S. cerevisiae) antibody ATG10 autophagy related 10 homolog antibody ATG10_HUMAN antibody autophagy 10, S. cerevisiae, homolog of antibody Autophagy related protein 10 antibody Autophagy-related protein 10 antibody DKFZP586I0418 antibody FLJ13954 antibody pp12616 antibody Ubiquitin-like-conjugating enzyme ATG10 antibody |
| Product description | Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is ATG10, an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist. |
| Immunogen | Purified recombinant fragment of human ATG10 (AA: 1-125) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | Apg 10 antibody
APG 10L antibody
APG10 antibody
APG10 autophagy 10 like antibody
APG10 like antibody
APG10 S. cerevisiae homolog of antibody APG10-like antibody APG10L antibody ATG 10 antibody ATG10 antibody ATG10 autophagy related 10 homolog (S. cerevisiae) antibody ATG10 autophagy related 10 homolog antibody ATG10_HUMAN antibody autophagy 10 S. cerevisiae homolog of antibody Autophagy related protein 10 antibody Autophagy-related protein 10 antibody DKFZP586I0418 antibody FLJ13954 antibody pp12616 antibody Ubiquitin-like-conjugating enzyme ATG10 antibody |
|---|---|
| Molecular Weight(MW) | 25.3kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9H0Y0 |
|---|
Application
Western blot analysis of ATG10 against human ATG10 (AA: 1-125) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of ATG10 against HEK293 (1) and ATG10 (AA: 1-125)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of ATG10 against K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of ATG10 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-38, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | K562 cell lysate, Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
