Application
Western blot analysis of MCM3AP against human MCM3AP (AA: extra 293-442) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of MCM3AP against HEK293 (1) and MCM3AP (AA: extra 293-442)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Product Name | MCM3AP [5B2C4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. |
| Immunogen | Purified recombinant fragment of human MCM3AP (AA: 293-442) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Purification | Unpurified |
| Alternative Names | 80 kDa MCM3 associated protein antibody 80 kDa MCM3-associated protein antibody FLJ44336 antibody FLJ45306 antibody Germinal center associated nuclear protein antibody KIAA0572 antibody MAP80 antibody MCM3 import protein antibody MCM3 minichromosome maintenance deficient 3 associated protein antibody MCM3A antibody MCM3A_HUMAN antibody Mcm3ap antibody Minichromosome maintenance complex component 3 associated protein antibody Minichromosome maintenance deficient 3 associated protein antibody Minichromosome maintenance protein 3 associated protein antibody Protein GANP antibody SAC3 antibody |
|---|---|
| Molecular Weight(MW) | 218.4kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | O60318 |
|---|
Application
Western blot analysis of MCM3AP against human MCM3AP (AA: extra 293-442) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of MCM3AP against HEK293 (1) and MCM3AP (AA: extra 293-442)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.| Positive Control | COS7 cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
