Application
Western blot analysis of DFFB against human DFFB (AA: 1-289) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Western blot analysis of DFFB against HEK293 (1) and DFFB (AA: 1-289)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Application
Flow cytometric analysis of DFFB was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-41, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | DFFB [2E6G3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAD antibody Caspase activated deoxyribonuclease antibody Caspase activated DNase antibody Caspase activated nuclease antibody Caspase-activated deoxyribonuclease antibody Caspase-activated DNase antibody Caspase-activated nuclease antibody CPAN antibody Deoxyribonuclease III, caspase activated antibody DFF 40 antibody DFF-40 antibody DFF2 antibody DFF40 antibody Dffb antibody DFFB_HUMAN antibody Didff antibody DNA fragmentation factor 40 kDa subunit antibody DNA fragmentation factor subunit beta antibody DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody DNA fragmentation factor, 40 kD beta subunit antibody DNA fragmentation factor, 40 kD, beta polypeptide antibody DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody OTTHUMP00000003633 antibody |
| Product description | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. |
| Immunogen | Purified recombinant fragment of human DFFB (AA: 1-289) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/mL |
| Alternative Names | CAD antibody
Caspase activated deoxyribonuclease antibody
Caspase activated DNase antibody
Caspase activated nuclease antibody
Caspase-activated deoxyribonuclease antibody
Caspase-activated DNase antibody
Caspase-activated nuclease antibody
CPAN antibody
Deoxyribonuclease III caspase activated antibody DFF 40 antibody DFF-40 antibody DFF2 antibody DFF40 antibody Dffb antibody DFFB_HUMAN antibody Didff antibody DNA fragmentation factor 40 kDa subunit antibody DNA fragmentation factor subunit beta antibody DNA fragmentation factor 40 Da beta polypeptide (caspase activated DNase) antibody DNA fragmentation factor 40 kD beta subunit antibody DNA fragmentation factor 40 kD beta polypeptide antibody DNA fragmentation factor 40kDa beta polypeptide (caspase activated DNase) antibody OTTHUMP00000003633 antibody |
|---|---|
| Molecular Weight(MW) | 39.1kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | O76075 |
|---|
Application
Western blot analysis of DFFB against human DFFB (AA: 1-289) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Western blot analysis of DFFB against HEK293 (1) and DFFB (AA: 1-289)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Flow cytometric analysis of DFFB was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1712-41, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Positive Control | Hela cells |
|---|---|
| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS with 0.05% sodium azide. |
