Application
Fig1: Western blot analysis of HLA Class 1 ABC on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: ICC staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HLA Class 1 ABC at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1801-10) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1801-10) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1801-10) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.| Product Name | HLA Class 1 ABC [11C2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen presenting molecule antibody HLA class I histocompatibility antigen, A 1 alpha chain antibody HLAA antibody Leukocyte antigen class I A antibody Major histocompatibility complex, class I, A antibody MHC class I antigen HLA A heavy chain antibody AS antibody HLA B protein antibody HLA class I histocompatibility antigen, B alpha chain antibody HLA-B antibody HLA-B histocompatibility type antibody HLAB antibody leukocyte antigen class I-B antibody lymphocyte antigen antibody major histocompatibility complex, class I, B antibody MHC class I antigen GN00104 antibody MHC class I antigen HLA-B heavy chain antibody MHC class I antigen SHCHA antibody MHC Class I HLA heavy chain antibody MHC HLA-B cell surface glycoprotein antibody MHC HLA-B transmembrane glycoprotein antibody SPDA1 antibody 1C07_HUMAN antibody Cw-7 alpha chain antibody D6S204 antibody HLA class I histocompatibility antigen antibody HLA class I histocompatibility antigen, C alpha chain antibody HLA class I histocompatibility antigen, Cw-1 alpha chain antibody HLA JY3 antibody HLA-C antibody HLA-C histocompatibility type antibody HLA-Cw antibody HLA-JY3 antibody HLAC antibody HLC C antibody HLC-C antibody human leukocyte antigen-C alpha chain antibody major histocompatibility antigen HLA-C antibody major histocompatibility complex, class I, C antibody MHC class I antigen antibody MHC class I antigen Cw 1 antibody MHC class I antigen Cw*7 antibody MHC class I antigen heavy chain HLA-C antibody PSORS1 antibody |
| Product description | HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. |
| Immunogen | Recombinant protein within Human HLA Class 1 ABC aa 120-310. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:500-1:2,000 ICC:1:50: IHC:1:50-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Antigen presenting molecule antibody
HLA class I histocompatibility antigen A 1 alpha chain antibody HLAA antibody Leukocyte antigen class I A antibody Major histocompatibility complex class I A antibody MHC class I antigen HLA A heavy chain antibody AS antibody HLA B protein antibody HLA class I histocompatibility antigen B alpha chain antibody HLA-B antibody HLA-B histocompatibility type antibody HLAB antibody leukocyte antigen class I-B antibody lymphocyte antigen antibody major histocompatibility complex class I B antibody MHC class I antigen GN00104 antibody MHC class I antigen HLA-B heavy chain antibody MHC class I antigen SHCHA antibody MHC Class I HLA heavy chain antibody MHC HLA-B cell surface glycoprotein antibody MHC HLA-B transmembrane glycoprotein antibody SPDA1 antibody 1C07_HUMAN antibody Cw-7 alpha chain antibody D6S204 antibody HLA class I histocompatibility antigen antibody HLA class I histocompatibility antigen C alpha chain antibody HLA class I histocompatibility antigen Cw-1 alpha chain antibody HLA JY3 antibody HLA-C antibody HLA-C histocompatibility type antibody HLA-Cw antibody HLA-JY3 antibody HLAC antibody HLC C antibody HLC-C antibody human leukocyte antigen-C alpha chain antibody major histocompatibility antigen HLA-C antibody major histocompatibility complex class I C antibody MHC class I antigen antibody MHC class I antigen Cw 1 antibody MHC class I antigen Cw*7 antibody MHC class I antigen heavy chain HLA-C antibody PSORS1 antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of HLA Class 1 ABC on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: ICC staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HLA Class 1 ABC at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1801-10) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1801-10) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HLA Class 1 ABC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1801-10) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.| Positive Control | Hela, rat uterus tissue, human kidney tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50: IHC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
